Review

human alveolar type-ii pneumocyte cell line a549 (Bioarray Inc)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Bioarray Inc human alveolar type-ii pneumocyte cell line a549

Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II <t>A549</t> cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Human Alveolar Type Ii Pneumocyte Cell Line A549, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+alveolar+type-ii+pneumocyte+cell+line+a549/pmc07851407-46-0-9?v=Bioarray+Inc
Average 90 stars, based on 1 article reviews

human alveolar type-ii pneumocyte cell line a549 - by Bioz Stars, 2026-08

90/100 stars

Images

1) Product Images from "Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19"

Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

Journal: Clinical Science (London, England : 1979)

doi: 10.1042/CS20201511

Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Figure Legend Snippet: Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Techniques Used: Activity Assay, Confocal Microscopy

Levels of proinflammatory cytokines (TNF-α, IL-6, CCL-2; A–C ) and ADAM17 enzymatic activity ( D and E ) in culture medium (A – C) and cells (D and E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test).

Figure Legend Snippet: Levels of proinflammatory cytokines (TNF-α, IL-6, CCL-2; A–C ) and ADAM17 enzymatic activity ( D and E ) in culture medium (A – C) and cells (D and E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test).

Techniques Used: Activity Assay

Image Search Results

M. leprae enters the alveolar epithelial cell lineage A549. Alveolar epithelial cells were infected with live M. leprae at an MOI of 10 at 33°C at increasing incubation times. The bacteria were labeled with PKH26 (red), and the cells were stained with DAPI (blue) and FITC-phalloidin (green). (A) Representative fluorescent image of 4 independent experiments showing live M. leprae bacilli interacting with A549 cells at 24 h of infection. (B, C, and D) Numbers of bacteria that interact with (B), adhere to (C), and enter (D) alveolar epithelial cells. (E) Percentages of bacterium-associated cells. Data represent the means ± standard deviations of the results of 4 experiments performed in duplicate. *, P < 0.05 was considered statistically significant.

Journal: Infection and Immunity

Article Title: Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

doi: 10.1128/IAI.00147-13

Figure Lengend Snippet: M. leprae enters the alveolar epithelial cell lineage A549. Alveolar epithelial cells were infected with live M. leprae at an MOI of 10 at 33°C at increasing incubation times. The bacteria were labeled with PKH26 (red), and the cells were stained with DAPI (blue) and FITC-phalloidin (green). (A) Representative fluorescent image of 4 independent experiments showing live M. leprae bacilli interacting with A549 cells at 24 h of infection. (B, C, and D) Numbers of bacteria that interact with (B), adhere to (C), and enter (D) alveolar epithelial cells. (E) Percentages of bacterium-associated cells. Data represent the means ± standard deviations of the results of 4 experiments performed in duplicate. *, P < 0.05 was considered statistically significant.

Article Snippet: The A549 human alveolar pneumocyte type II cell line (ATCC CCL185; ATCC, Manassas, VA) was cultured in Dulbecco's modified Eagle's medium (DMEM)-F12 (ATCC) supplemented with 10% (vol/vol) serum-inactivated fetal bovine serum (FBS; Gibco, Grand Island, NY).

Techniques: Infection, Incubation, Labeling, Staining

Transmission electron micrographs of M. leprae-infected epithelial cells. Epithelial cells were infected with live M. leprae for 24 h at 33°C. RPMI 2650 (A and C) and A549 (B and D) cells were fixed, processed, and visualized by transmission electron microscopy. The arrows point to bacteria; the arrowheads point to the membrane-bound compartments.

Journal: Infection and Immunity

Article Title: Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

doi: 10.1128/IAI.00147-13

Figure Lengend Snippet: Transmission electron micrographs of M. leprae-infected epithelial cells. Epithelial cells were infected with live M. leprae for 24 h at 33°C. RPMI 2650 (A and C) and A549 (B and D) cells were fixed, processed, and visualized by transmission electron microscopy. The arrows point to bacteria; the arrowheads point to the membrane-bound compartments.

Techniques: Transmission Assay, Infection, Electron Microscopy

M. leprae enters airway epithelial cells in a passive cytoskeleton-dependent manner. (A to D) Live and heat-killed M. leprae interaction with RPMI 2650 (A and C) and A549 (B and D) cells for 24 h at 33°C. The bacteria were labeled with PKH26, and the numbers of bacteria interacting with the cells and the percentages of bacterium-associated cells were determined by fluorescence microscopy. (E and F) Effect of cytoskeleton inhibitors on M. leprae uptake by RPMI 2650 cells (E) and A549 cells (F). Cells were pretreated with DMSO (drug vehicle), colchicine, or cytochalasin B for 1 h and during the 24 h of the assay at 33°C. Data represent the means ± standard deviations of the results of 3 experiments performed in duplicate. **, P < 0.01.

Journal: Infection and Immunity

Article Title: Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

doi: 10.1128/IAI.00147-13

Figure Lengend Snippet: M. leprae enters airway epithelial cells in a passive cytoskeleton-dependent manner. (A to D) Live and heat-killed M. leprae interaction with RPMI 2650 (A and C) and A549 (B and D) cells for 24 h at 33°C. The bacteria were labeled with PKH26, and the numbers of bacteria interacting with the cells and the percentages of bacterium-associated cells were determined by fluorescence microscopy. (E and F) Effect of cytoskeleton inhibitors on M. leprae uptake by RPMI 2650 cells (E) and A549 cells (F). Cells were pretreated with DMSO (drug vehicle), colchicine, or cytochalasin B for 1 h and during the 24 h of the assay at 33°C. Data represent the means ± standard deviations of the results of 3 experiments performed in duplicate. **, P < 0.01.

Techniques: Labeling, Fluorescence, Microscopy

M. leprae survives inside epithelial cells. Intra- and extracellular M. leprae viability in RPMI 2650 and A549 cells was determined by the use of a Live/Dead kit. (A) RPMI 2650 cells at 33°C. (B) A549 cells at 33°C. (C) A549 cells at 37°C. Intracellular M. leprae bacilli (black bars) were recovered by lysing buffer, while extracellular bacteria (gray bars) were recovered after washing steps. In parallel, M. leprae bacilli were incubated in cell-free medium (white bar). Bacteria were stained by the use of a Live/Dead BacLight Bacterial Viability kit and enumerated by direct counting via fluorescence microscopy at a magnification of ×1,000. The data represent the mean values ± SD of the results of five experiments with duplicate samples from each one. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Journal: Infection and Immunity

Article Title: Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

doi: 10.1128/IAI.00147-13

Figure Lengend Snippet: M. leprae survives inside epithelial cells. Intra- and extracellular M. leprae viability in RPMI 2650 and A549 cells was determined by the use of a Live/Dead kit. (A) RPMI 2650 cells at 33°C. (B) A549 cells at 33°C. (C) A549 cells at 37°C. Intracellular M. leprae bacilli (black bars) were recovered by lysing buffer, while extracellular bacteria (gray bars) were recovered after washing steps. In parallel, M. leprae bacilli were incubated in cell-free medium (white bar). Bacteria were stained by the use of a Live/Dead BacLight Bacterial Viability kit and enumerated by direct counting via fluorescence microscopy at a magnification of ×1,000. The data represent the mean values ± SD of the results of five experiments with duplicate samples from each one. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Techniques: Incubation, Staining, Fluorescence, Microscopy

M. leprae recombinant Hlp and HBHA mediate the mycobacterial attachment to airway epithelial cells. (A and B) Fluorescent beads coated with Hlp (A), with HBHA (B), or with BSA (negative control) were incubated with RPMI 2650 and A549 cells for 1 h at 37°C. Cells were then washed and fixed. The numbers of beads associated with these cells were counted in a fluorescence microscope in a set of 300 cells using a magnification of ×400. (C) Fluorescent beads coated with Hlp, HBHA, or BSA were incubated simultaneously with A549 cells for 1 h at 37°C. (D) M. smegmatis (MS) was pretreated or not with Hlp (MS+Hlp) or HBHA (MS+HBHA), and an ELISA was performed with anti-Hlp or anti-HBHA, respectively, in order to monitor the binding of these proteins to the bacterial surface. (E) MS and Hlp or MS and HBHA were used to infect epithelial cells for 2 h at 37°C. Internalized bacteria were released with lysis buffer for CFU counting. The data represent the mean values ± SD of the results of at least three experiments performed in duplicate. *, P < 0.05; **, P < 0.01.

Journal: Infection and Immunity

Article Title: Interaction of Mycobacterium leprae with Human Airway Epithelial Cells: Adherence, Entry, Survival, and Identification of Potential Adhesins by Surface Proteome Analysis

doi: 10.1128/IAI.00147-13

Figure Lengend Snippet: M. leprae recombinant Hlp and HBHA mediate the mycobacterial attachment to airway epithelial cells. (A and B) Fluorescent beads coated with Hlp (A), with HBHA (B), or with BSA (negative control) were incubated with RPMI 2650 and A549 cells for 1 h at 37°C. Cells were then washed and fixed. The numbers of beads associated with these cells were counted in a fluorescence microscope in a set of 300 cells using a magnification of ×400. (C) Fluorescent beads coated with Hlp, HBHA, or BSA were incubated simultaneously with A549 cells for 1 h at 37°C. (D) M. smegmatis (MS) was pretreated or not with Hlp (MS+Hlp) or HBHA (MS+HBHA), and an ELISA was performed with anti-Hlp or anti-HBHA, respectively, in order to monitor the binding of these proteins to the bacterial surface. (E) MS and Hlp or MS and HBHA were used to infect epithelial cells for 2 h at 37°C. Internalized bacteria were released with lysis buffer for CFU counting. The data represent the mean values ± SD of the results of at least three experiments performed in duplicate. *, P < 0.05; **, P < 0.01.

Techniques: Recombinant, Negative Control, Incubation, Fluorescence, Microscopy, Enzyme-linked Immunosorbent Assay, Binding Assay, Lysis

Journal: Clinical Science (London, England : 1979)

Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

doi: 10.1042/CS20201511

Figure Lengend Snippet: Levels of ACE2 protein ( A,D and E ) and enzymatic activity ( B and C ) in cells (A and D) and culture medium (E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. Laser confocal microscopy showing colocalization ( H , yellow) of ACE2 (green, F ) and spike protein (red, G ) in cells after treatment of cultures with spike protein. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls Method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test); scale bar (for F – H): 25 µm.

Article Snippet: Human alveolar type-II pneumocyte cell line A549 (CSC-C6236X, Creative Bioarray) was cultured in DMEM/F12 medium supplemented with 10% FBS, 2 mM L-glutamine (Sigma), 100 U/ml penicillin and 100 μg/ml Streptomycin.

Techniques: Activity Assay, Confocal Microscopy

Journal: Clinical Science (London, England : 1979)

Article Title: Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19

doi: 10.1042/CS20201511

Figure Lengend Snippet: Levels of proinflammatory cytokines (TNF-α, IL-6, CCL-2; A–C ) and ADAM17 enzymatic activity ( D and E ) in culture medium (A – C) and cells (D and E) in cultures of human alveolar type-II A549 cells treated with spike protein alone or spike protein together with candesartan or captopril. * P <0.05 relative to untreated controls; # P <0.05 relative to cells treated with spike alone (one-way ANOVA with Student–Newman–Keuls method post hoc test or Kruskal–Wallis one-way analysis of variance on ranks with Student–Newman–Keuls method post hoc test).

Techniques: Activity Assay

Review

Structured Review

Images

1) Product Images from "Experimental data using candesartan and captopril indicate no double-edged sword effect in COVID-19"

Similar Products

Image Search Results